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The present study was performed to evaluate the enhancing effect of periodontal healing by ascorbic acid and unsaponifiable fractions of Zea Mays L. on the experimentally induced periodontitis in rats by mechanical irritation. One hundred twenty male Sprague-Dawley rats, weighing 100 - 120gms, were utilized as experimental animals. Experimental animals were divided into four experimental groups and two controls. Each experimental group was composed of 25 rats and each control was 10 rats respectively. Experimental periodontitis was induced by mechanical irritation. This irritation was performed with sharp instrument on the interdental papillae of rat´s upper right molars every other day for two weeks. Experimental animals received orally ascorbic acid by 1 mg/ I OOgm of body weight and/or Zea Mays L. by O.Sml/ 100gm of body weight in mixture of edible oil daily. Experimental animals were grouped as follows: Group I: ascorbic acid administered group in periodontitis state without continuing irritation. Group II: Zea Mays L. administered group in periodontitis state without continuing irritation. Group III: ascorbic acid and Zea Mays L. administered group in periodontitis state without continuing irritation. Group IV ascorbic acid and Zea Mays L. administered group in periodontitis state with continuing irritation. Group V. no treatment group in periodontitis state without continuing irritation. Group VI: ascorbic acid and Zea Mays L. administered group in normal state without continuing irritation. After the development of experimental periodontitis, ascorbic acid and/or Zea Mays L. was administered. The rats were sacrificed on the 1st, 3rd, 7th, 10th, 14th, and 21th day. The experimental regions were surgically excised out and routinely processed for microscopic examination. The specimen were stained with Hematoxylin-Eosin.
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